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Jeff L De Jong
Associate Professor-Biological Sciences
Office MailstopMail Box: RL11, Room No.: RL1704 
Email Address    Primary Phone Number 972-883-6882    URL Jeff De Jong's Web Page    Media Contact
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Square, Eloise
 Professional Preparation
  Molecular BiologyRockefeller University1990-1995
 Ph.D.BiochemistryPennsylvania State University1990
 B.A.BiologyUniversity of Missouri-Columbia1984
Collapse Section Expand Section Research and Expertise
Research Interests
In order for a gene to be expressed, a preinitiation complex composed of RNA polymerase II, general transcription factors (GTFs) TFIIA, -B, -D, -E, -F, and –H, and other factors must be assembled.  The level of RNA produced is then determined by the action of gene-specific transcriptional activators or repressors and their associated cofactors.
One step in the assembly of a preinitiation complex is the recognition of core promoter DNA. This step is the primary responsibility of a family of TBP related factors including TBP, TRF1, TRF2, and TRF3. The interactions of these factors with DNA is assisted by TFIIA and ALF, and their stability is an important determinant of the rate of transcription.
Results from this lab and others have shown that distinct types of core promoter and DNA packaging factors are expressed in reproductive tissues of humans, mice, and frogs. These observations suggest there are three distinct transcription environments—somatic, spermatogenic, and oogenic—each with their own collection of core promoter factors. Further differences in the phylogenetic distribution of individual germ cell transcription factors suggest these systems are adapted to species-specific variations in reproductive physiology. Overall, the expression of transcription factor variants in reproductive tissues is believed to be important for male- or female-specific patterns of gene expression and for completion of gametogenesis. Current work in the laboratory continues to explore connections between the biochemical mechanisms of transcription, the biology of germ cells, and reproductive health.
Collapse Section Expand Section Publications
 1  2  
  YearPublication  Type
Upadyhaha, A.B. and DeJong J. Expression of human TFIIA subunits in S. cerevisiae identifies regions with conserved and species-specific functions. Biochemica Biophysica Acta (in press).
Category: Biochemica Biophysica Acta
Peer reviewed
Xie, W., Han, S.Y., Khan,M., and DeJong, J. (2002) Regulation of ALF gene exprssion in somatic and male germ line tissues involves partial and site-specific patterns of methylation. J. Biol. Chem. 277: 17765-17774.
Category: The Journal of Biological Chemistry
Peer reviewed
Upadhyaya, A., Khan, M., Mou, T.-C., Junker, M., Gray, D.M., and DeJong, J. (2002) The germ cell-specific transcription factor ALF: Structural properties and stabilization of TBP-DNA complexes. J. Biol. Chem. 277: 34208-34216.
Category: The Journal of Biological Chemistry
Peer reviewed
Han, S.Y., Zhou, L.P., Upadhyaya, A., Lee, S.H., Parker, K.L., and DeJong, J. (2001) TFIIAa/b-like Factor (ALF) is encoded by a germ cell-speific gene whose expression is upregulated with other general transcription factors during spermatogenesis in mouse. Biol. Reprod. 64: 507-517.
Category: Biology of Reproduction
Peer reviewed
Lee, S.H., Wang, X.L., and DeJong, J. (2000) Functional interactions between an atypical NF-kB stie from the rat CYP2B1 promoter and the transcriptional repressor RBP-Jk/CBF1. Nuc. Acids Res. 28: 2091-2098.
Category: Nucleic Acids Research
Peer reviewed
Collapse Section Expand Section Affiliations
Dr. Keith Parker, UT-Southwestern
Dr. Steve Hammes, UT-Southwestern
Graduate and Postdoctoral Advisors
Dr. Chen-Pei David Tu (graduate)
Dr. Robert G. Roeder (post-doc)
Thesis Advisor and Postgraduate-Scholar Sponsor
1995-2000 SangHyun Lee (Ph.D. completed)
1997-2002 Ashok Upadhyaya (Ph.D. completed)
1998-- SangYoon Han (Current Ph.D. candidate)
1995-1996 Dr. Xiao-li Wang (past postdoctoral associate)
2000-2002 Dr. Wensheng Xie (past postdoctoral associate)
Collapse Section Expand Section Appointments
DurationRankDepartment / SchoolCollege / OfficeUniversity / Company
2001-PresentAssistant Professor  University of Texas at Dallas
1995-2001Assistant Professor  University of Texas at Dallas
1990-1995Postdoctoral Associate  The Rockefeller University
1984-1990Teaching Assistant  Pennsylvania State University
 Synergistic Activities
Synergistic Activities
1986-1987    One year as Visiting Graduate Student, Academia Sinica, Tapei, Taiwan
1999               Invited junior faculty participant at the American Cancer Society Research Professors Meeting

1995---           American Association for the Advancement of Science (AAAS)
1999---           Texas Faculty Association (TFA)

Undergraduate Students
Hau Hou
Jennifer Peace
Jennifer Wabin           UT-Southwestern Medical School
Mohammed Khan      Texas Tech Medical School

Courses Taught
Biology 5410    (50%; Fall) Biochemistry of Proteins and Nucleic Acids
Biology 6329    (50%; Spring) Regulation of Gene Expression in Eubacteria and Eukaryotes
Biology 3362    (33%; Spring) Biochemistry II
Biology 5420    (25%; Fall) Molecular Biology
Biology 5V51    (33%; Various) Methods in Molecular and Cell Biology II

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