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    Faculty Profile — Is this you? Login to edit.Last Modified Time: 09:42:43 AM Thu, 8 Nov 2012 
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Irina G Borovkov
Senior Lecturer I-Natural Sciences & Mathematics, School of
Office MailstopMail Box: FO31 
Email Address  ixb053000@utdallas.edu    Primary Phone Number 972-883-6895    Media Contact
 Professional Preparation
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 DegreeMajorInstitutionYear
 Ph.D.GeneticsUSSR Academy of Sciences, Moscow, Russia 
 B.S.Plant and Animal Genetics and CytologyNovosibirsk State University, Russia 
 M.S.Plant and Animal Genetics and CytologyNovosibirsk State University, Russia 
Collapse Section Expand Section Publications
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  YearPublication  Type
2000
Graner A., Streng S., Drescher, A., Jin Y., Borovkova I., and Steffenson B.J. Molecular mapping of the leaf rust resistance gene Rph7 in barley. Plant Breeding, 119, 389-392, 2000.
Category: Plant Breeding
Peer reviewed
2000
Borovkov I., Jennings, J .C., and Lirette R.P. Confirmation of the genomic DNA sequences flanking the 5' and the 3' ends of the insert in YieldGard Corn Event Mon 810. MSL-16776, Sept. 6, 2000
 
2000
Lirette R.P., Cavato, T.A., Reiser, S.E., Beazley, K. A., Borovkov I.G., and Higgins, M. R. DNA Sequence Determination of the 72 bp CP4 EPSPS DNA Insert and the Flanking Soybean Genomic DNA in the Roundup Ready Soybean Event 40-3-2. MSL-16727, Jrme 5, 2000.
 
2000
Lirette R.P., Cavato, T.A., Reiser, S.E., Beazley, K. A., Borovkov I.G., Higgins, M. R., Albee, L.D., and Dixon, D.A. Further molecular characterization of Rormdup Ready Soybean Event 40-3-2. MSL-16646, May 12, 2000.
 
2000
Jemiings, J .C., Borovkov I.G., and Lirette, R.P. Molecular Analysis of com event MON 849. MSL-16080, April, 4, 2000.
 
Collapse Section Expand Section Presentations and Projects
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Start DateEnd DatePresentation/Project
1997 1997 Chromosomal location and genetic relationship of the leaf rust resistance genes Rph9 and Rph12 in barley.
Borovkova I.G., Jin, Y., and Steffenson, B.J. 1997. Plant and Animal. Genome V, San Diego, California.
1997 1997 Analysis and mapping of disease resistance related sequences in common bean (Phaseolus vulgaris) and barley (Hordeum vulgare).
Rivkin, M.I., Borovkova I.G., Borovkov, A.Y., Steffenson, B.J., McClean P. 1997. Plant and Animal .Genome V, San Diego, Califomia.
1995 1995 Molecular markers for transferring multiple rust resistance genes in barley.
Borovkova I. G., Steffenson B. J., Jin Y., Rasmussen J. B., Kilian A., Kleinhofs A. 1995. Molecular markers for transferring multiple rust resistance genes in barley. Plant Genome III, San Diego, California.
1994 1994 Molecular marking of the stem rust resistance gene rpg4 in barley
Borovkova I. G., Jin Y., Steffenson B. J., Rasmussen J. B. 1994. APS annual meeting, Albuquerque, New Mexico.
1993 1993 Identification of RAPD markers linked with disease resistance in barley.
Borovkova I. G., Steffenson B. J., Rasmussen J. B., Jin Y., Rossnagel B. G., Kao K. 1993.APS annual meeting, Fargo, North Dakota.
Collapse Section Expand Section Appointments
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DurationRankDepartment / SchoolCollege / OfficeUniversity / Company
1999-2002Molecular analyst (Principal investigator)Regulatory Sciences Monsanto Co., St. Louis, MO
1997-1999Molecular geneticistDisease control Monsanto Co., St. Louis, MO
1992-1997Research AssociateDepartment of Plant Patholog North Dakota State University, Fargo, ND
1991-1992Visiting ScientistDepartment of Crop and Weed Sciences North Dakota State University, Fargo, ND
01/2003-07/2003Molecular AnalystGenomics and Biotechnology Group Pfizer, St. Louis, MO
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 Additional Information
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Qualifications Summary
Creative, goal-oriented professional with demonstrated experience in a variety of molecular biology and genetics techniques. Author of more than twenty publications. Highly organized with the ability to manage multiple projects and meet deadlines. A team player, effectively designing new research projects, troubleshooting and interpreting experimental results. Good communication and presentation skills. Computer literate and trained in DNA analysis bio-informatics tools. 

Professional Skills
  • Molecular biology:  Experienced in the most recent molecular biological methods:  DNA and RNA isolation; Southern and Northern blot analysis. DNA and protein analysis methods, different types of electrophoresis, including F IGE; gel image analysis. DNA probe construction; molecular cloning and library (bacterial, lambda and cosmid) construction, dot, blot and colony hybridizations; radioactive and nonradioactive DNA labeling and detection; site-directed mutagenesis; Gateway Cloning Technology. 3'- and 5'- RACE, RT-PCR, PCR amplification, product analysis and cloning, genome walking, TAIL PCR, inverse PCR, real-time PCR (TaqMan, Opticon), fluorescent probes. Molecular markers (RFLPs, AF LP, microsatellites, RAPDs).
  • Computer skills: Apple, IBM operative systems. Database sequence searching (Incyte, NCBI, national plant genome databases). DNA analysis: GCG Wisconsin Package, Sequencher, DNA Strider; DNAStar, PrimerSelect (trained in sequence analysis at Monsanto internal classes and Monsanto Sequencing Center seminars). Proficient in genetic map constructing software such as MapMaker, .IoinMap, G-Mendel. Microsoft Office 2000 and NT (Word, Excel, PowerPoint).

Professional Experience
Molecular analyst, Genomics and Biotechnology Group January-July, 2003
Performed site-directed mutagenesis and cloned DNA sequences for fimctional analysis and expression in multiple systems for oncology and osteoporosis studies. Monsanto Co., St. Louis, MO
Molecular analyst (Principal investigator), Regulatory Sciences 1999-2002
Directed molecular characterization of transgenic events for regulatory approval of federal agencies. Implemented research plans in the specific project area. Coordinated work of other team members under pressing timelines. Build putative sequence and restriction map of a near 100 kb genomic region containing a transgene cassette and flanking genomic sequences of a maize transgenic event by using molecular markers, sequence comparison and BAC clones analysis. The generated map was essential for resolving complex regulatory issues and commercial line certification. Contributed to the European approval of Roundup Ready soybean and com lines by successfirl resolving molecular structure of complex transgene integration events. Developed a real time PCR assay for high tlnoughput analysis of transgenic lines seed purity evaluation. The assay has been implemented into the SOP for seed quality control.
Molecular geneticist, Disease control 1997-1999
Identified several soybean genes putatively responsible for disease resistance using homology search, EST analysis, transcriptional profiling, and other bio-informatics tools. Initiated and coordinated an interdepartmental effort for mapping, identification and cloning of plant genes involved in pathogen resistance. More efficient resource utilization led to a significant acceleration of a linkage map construction process and successful cloning of a resistance gene. Developed new molecular markers for disease resistant genes in barley for cloning purposes. North Dakota State University, Fargo, ND
Research Associate, Department of Plant Pathology 1992-1997
Performed mapping of the resistance genes in barley. By persistent use of different molecular markers was the first one to position three resistant genes on the barley map. Received certificate of recognition from the Department for scientific achievements. Managed molecular biology laboratory,supervised and trained technical personnel and graduate students.
Visiting Scientist, Department of Crop and Weed Sciences 1991-1992
Developed molecular markers (RAPD and RFLP) for identification and distinction of sunflower downy mildew (Plasmnpara halstedii) races. Improved DNA extraction method for Plasmopam halstedii and was the first one to use RAPD markers for its races identification. 

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